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How to Extract DNA

1. Chop the onion into small pieces and place inside the blender container.

Chopped onion
magnifyChopped onion


2. Measure out 200 mls of the water. Pour this into a glass and add one level teaspoon of salt. Microwave the salty water for one minute (High) then stir well.

Add salt to water
magnifyAdd salt to water


3. Add enough of the salty water to just cover the onion.

Add salty water and onion together
magnifyAdd salty water to onion


4. Blend the mixture for about 10 seconds.

Blend mixture
magnifyBlend mixture


5. Pour the onion-salt-water mixture through the strainer and collect the liquid in a glass. The DNA from the onion is in this liquid.

Strain liquid
magnifyStrain liquid


6. Measure out 50 mls of the onion liquid and pour it into a glass.

Measure liquid
magnifyMeasure liquid


7. Add 2 teaspoons of dishwashing detergent to the glass containing the 50mls of onion liquid. Stir very gently.

Add detergent
magnifyAdd detergent


8. Measure out 100 mls of methylated spirits. Slowly add the methylated spirits to the glass containing the onion-detergent mixture.

Add methylated spirits
magnifyAdd methylated spirits


9. Now wait for the DNA to appear! The DNA is white, rising up from the bottom of the glass. Be patient as it may take a few minutes to appear. (If no DNA appears after 5 minutes, try again using suggestions from What went wrong? )


The stages of the experiment

DNA rising from the bottom of the cup to the top


Clean up the kitchen. The methylated spirits and DNA from your experiment should be poured down the sink.



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